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KMID : 0043319900130010033
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Archives of Pharmacal Research
1990 Volume.13 No. 1 p.33 ~ p.37
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Activity and Stability of Purified Amylase Produced by Streptomyces aureofaciens 77
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Ibrahim AN
Ibrahim MMK/Ahmed FH/Arafa MAI
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Abstract
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The effects of pH values, temeprature and some elements on the amylolytic activity and stability of the purified S. aureofaciens 77 amylase were studied in this investigation. The purified enzyme showed its maximum activity at pH 6 within 8 min incubation at 40oC. None of the tested 6 metals showed on stimulatory effect on the enzymatic activity,Fe+++,Cu++ and Hg++ at high dose inhibited the enzyme activity to great extent as compared with Zn++, Mn++ and Fe++ which gave less effect in this respect. The enzyme liquor was found to be thermolabile, since it lost completely its activity after 4 days incubation under room temperature and showed maximum activity during this period as a result of additions of Ca++ and NaCl. Gradual reduction was however recorded until activity reached 30% after 60 days of incubation.
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